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The main objective of this study is to clarify the impact of the over expressing protein p7. Cells used had completed the amitotic stage but not the cytokinesis. Since the protein was previously shown to bind GFP and myosin of Tetrahymena thermophila, it was immunostained with anti-myo1 to acquire immunofluoresence images. Macronucleous undergoes amitosis where the intramacronulcear microtubules form lines. It is common in MYO1 and in overexpression of GFP-tagged MYO1 that these lines don't work properly in macronuclear elongation which lead to unequal division of macronucleus. Although, there is co localization of GFP-p7 with antitubulin to intramacronuclear microtubules, it is not regulated enough. At the beginning of GFP-P7 over expression the cells are directed randomly on the intramacronuclear microtubules, they don’t form the parallel array since the macronuclei did not achieve full elongation. Then the macronuclear division in overexpressiong cells was either unequal o r inhibited. Introduction The experiment is based on Myo1 which has a MyTH4 and a FERM domain. Myo1 knockout strain affects both mitosis and amitosis which occurs in Tetrahymena in the same cell but different nuclei. Some micronucleus divide with spindles fibers while others divide without spindle fibers. The intramacronuclear microtubules shape array directed parallel to the axis of macronuclear elongation in amitosis (Williams & Williams, 1976). Macronuclear elongates along with the elongation intramacronuclear microtubules. The elongated macronucleus can enlarge the length of the cell borders through cytoplamic microtubules. Subnuclei are formed due to constriction of nucleus at its midpoint. Although these parallel arrays work as a spindle, nu... ...peron is a major factor that determine normal or abnormal functioning of the protein upon its formation even though other processes referred to as post translational modification plays a necessary process (Aufderhide, 1979). From the observation below, the GFP tagged p7 colocalize within the live cells. Production of an intense GFP means that the protein aggregated more to the cell body. This means that the protein has an increased potential for the cell membrane. Analysis of the cells was based on Leitz wetzlar epifluoresence using LAS as software that gave the images shown below. A protein has a feature like cyclin-like, binds to GFP and myosin of Tetrahymena Thermophila. This study also compliments previous studies that in fact the molecular mass is 7 kDa, which has the ability of processing cyclin-like protein can bind to Myo1 and GFP (Ejercito & Wolfe, 2003).

Non-violent social change

Chavez, King Jr. and Malcolm X all advocated non-violent struggles for justice and equality. They were all successful in their policy goals. Through the tactics and strategies that they employed, they were able to achieve their goals. Through their leadership, the marches, boycotts, rallies, fasts and strikes led to their objectives. Non-violent struggles proved to be more effective in making people aware and bringing out desired changes.Their experiences also led them to be the leaders of their organizations. Chavez has seen a lot of inequality towards Mexican-Americans especially the farm workers. King Jr., on the other hand, has also experienced inequality and injustice even when he was young. And when he became the leader of the Southern Christian Leadership Conference (SCLC), he saw a lot of racial discrimination and inequality in rights against the blacks. Malcolm X, too, has experienced being harassed when he was young. In fact, his life before being a Muslim could be said as sorrowful. His father was killed, his mother was taken to a mental institution and he and his siblings were split up to different homes.Discrimination seemed to be the factor which drove these leaders to seek change. Chavez has encountered it when he was in elementary schools. King Jr. has seen discrimination against the blacks. Malcolm X has probably experienced discrimination as he talked about black pride. Due to this, they have become resolved in bringing social change and civil rights.I believe that their advocacy of non-violent social change played a big role in their success as leaders. As to Chavez and King, their mothers taught them that there should be no place for violence or any discrimination in one’s heart.These three leaders were long dead, but their legacy still lives. Malcolm X had influenced both the blacks and the whites. The blacks has regarded him as a man of the people and experienced in the ways of the street. The whites, on the other hand, were influen ced by his unwillingness to turn away from hostility. Chavez’s legacy, on the other hand, is his philosophy of service for others, justice, equality and civil rights. And King Jr. has brought the Voting Rights Act and the Civil Rights Act.WORKS CITEDMcElrath, Jessica. Malcolm X. Online. Available: http://afroamhistory.about.com/cs/malcolmx/a/bio_x_malcolm.htm. 30 September 2007.Siqueiros, Ray. â€Å"Who was Cesar Chavez?† People’s Weekly World. 19 Apr 2003: 11 pars. Online. Available: http://www.pww.org/article/articleview/3309/1/

Charlotte Bronte s Jane Eyre - 1371 Words

In a time period where women were unequal and unheard in society, Charlotte Brontà « expresses her feminist ideals through her novel Jane Eyre, an unexpected love story between an unlikely pair. During her work as a governess at Thornfield Hall, Jane gets to know her master, Mr. Rochester, very well and begins to fall in love with him. Jane at first forbids herself from a courtship because of the class difference between them; however, in time she learns that the feelings of love are mutual and they plan to marry. Unfortunately, a lawyer interrupts their wedding and claims Mr. Rochester has a living wife, which proves true when Mr. Rochester introduces Jane to Bertha, his insane spouse. Unwilling to be Rochester s mistress, Jane flees him and finds work, only to realize that her employer is her cousin and that her uncle left her with a large sum of money. Now equal in rank to Rochester, Jane seeks him out and finds out that his wife died burning Thornfield Hall to the ground, which means that Jane can finally marry Rochester. Although it cannot be classified as a gothic novel, Brontà « purposefully includes elements of Gothic literature to cause changes in the plot that identify feminist characteristics in the main character, Jane Eyre. Multiple supernatural occurrences certainly act as catalysts for changes that reveal Jane s independence. Places such as the red-room and events like Bertha s introduction and the answer to Jane’s prayer serve as stimuli for major plot shiftsShow MoreRelatedSummary Of Charlotte Bronte s Jane Eyre1607 Words   |  7 Pageswere expected to obey a man s commands and were treated inferior to their male superior. This novel embodies the ideology of equality between men and women in society. Charlotte Bronte s novel Jane Eyre embraces many views in opposition to the Victorian gender limitations. Ultimately, the reader can see the author develops a variety of characters who not only represent but also challenge the establishe d gender norms existing in the 1800s. Feminists like Charlotte Bronte, fight for equality emotionallyRead MoreAnalysis Of Charlotte Bronte s Jane Eyre 2054 Words   |  9 Pageswith the present and consumed by desire and materialism, resulting in their inevitable self-destruction. In Charlotte Brontà «Ã¢â‚¬â„¢s gothic novel, Jane Eyre, Brontà « creates a multitude of characters that represent the extremes of the id and the superego to contrast with Jane’s more balanced, ego-dominated self in order to reveal the dangers of falling out of balance within oneself. In doing so, Brontà « illustrates an aspect of the human condition that reveals that in order to maintain both inner and outerRead MoreMarxist Criticism On Charlotte Bronte s Jane Eyre1467 Words   |  6 PagesComposition March 18, 2016 Marxist Criticism on Charlotte Brontà « s Jane Eyre Some novels will not let the reader escape the social setting, and Charlotte Brontà « s Jane Eyre is no acceptation. The author implements a symphony of details that strikes the reader as a full blown portrait of society. The novel s surroundings profoundly influence the thoughts, emotions, and actions of every character, which makes out the setting to be as important to the novel s development as the characters and personalitiesRead MoreReview Of Charlotte Bronte s Jane Eyre 10879 Words   |  44 PagesNotes Jane Eyre Background of author Name: Charlotte Bronte Birth/Death: April 21, 1816 to March 31,1855 Facts that connect: Mr. Brocklehurst is based off the Reverend Carus Wilson, the man who ran Cowan Bridge. Bronte lost two of her sisters, Mary and Elizabeth, to tuberculosis at Cowan Bridge. Bronte s brother, Patrick, became addicted to drugs and alcohol before he died. Similarities: She, along with her three sisters, was sent to the Clergy Daughters School at Cowan Bridge. Charlotte BronteRead MoreCharlotte Bronte s Jane Eyre984 Words   |  4 PagesCharlotte Brontà « wrote Jane Eyre. The novel is a highly fascinating piece of gothic literature based during the nineteenth century. The story unfolds around a young Jane Eyre who grows both in character and body. She experiences love and mystery. She, through many trials, finds a true, wholesome, and godly relationship with the main male protagonist, Mr. Rochester. Jane Eyre occurs during the early nineteenth century in northern Britain. It takes place in multiple locations: â€Å"the Reed family’sRead MoreCharlotte Bronte s Jane Eyre1074 Words   |  5 PagesMatthew Fastiggi Jane Eyre Essay There is perhaps no human emotion that can evoke feelings of both utter despair and incredible joy like love can. Throughout the novel Jane Eyre by Charlotte Bronte, Jane searches tirelessly for love while living in a mostly cruel and heartless world. Jane’s journey goes from a childhood doll being the entirety of her love to marrying Mr. Rochester, the love of her life. This is symbolic of the massive change Jane undergoes throughout the novel and how love affectsRead MoreCharlotte Bronte s Jane Eyre1228 Words   |  5 Pagessuspense, Charlotte Bronte’s Jane Eyre is a novel that the term â€Å"gothic† could be applied to. You can find these elements in the setting, characters, and in numerous places in the plot of this novel. Thornfield Hall is the quintessential gothic castle. Most gothic novels settings include; a castle, ruined or intact, haunted or not. (The Gothic Experience 1). Thornfiled Hall seems to have a life or story of its own throughout the plot. The way it is described changes as the story progresses. Jane is quotedRead MoreEquality Within Charlotte Bronte s Jane Eyre1673 Words   |  7 Pageswere expected to obey a man s commands and were treated inferior to their male superior. This novel embodies the ideology of equality between men and women in society. Charlotte Bronte s novel Jane Eyre embraces many views in opposition to the Victorian gender limitations. Ultimately, the reader can see the author develops a variety of characters who not only represent but also challenge the established gender norms existing in the 1800s. Feminists like Charlotte Bronte, fight for equality emotionallyRead MoreAnalysis Of Charlotte Bronte s Jane Eyre1110 Words   |  5 Pagesconsidered equal to men. They have not been since the beginning and although we say they are now, many would probably disagree. Charlotte Brontà « was no exception to this rule- she knew that society expected women to fit into a specific category rather than be their own individuals and she expressed her frustration towards this in Jane Eyre. Within this brief passage, Charlotte Brontà « elegantly employs symbolism, allusions, and diction in order to assert Jane’s determination to stay true to herself, despiteRead MoreReview Of Charlotte Bronte s Jane Eyre 933 Words   |  4 PagesTitle: Jane Eyre Author: Charlotte Bronte Main Characters (Protagonist/Antagonist), Title, Traits: Jane Eyre (Protagonist): main character, lost, alone, devastated Rochester (Protagonist): master of Thornfield, loves Jane, secretive, caring St. John (Protagonist): missionary loves Jane, cold, reserved, controlling Mrs. Reed (Antagonist): Jane’s aunt, cruel, resent Mr. Brocklehurst (Antagonist): principal, cruel, hypocritical Setting: England in the 19th century. Gateshead, Lowood Institute